Figure 5

Effect of DDS on PQ-induced PKC activation in mouse lung tissue. PQ-induced PKC phosphorylation was evaluated by Western blotting. Mice were pretreated daily with oral DDS (0.5 or 2 mg/kg) for 1 week and then treated once with 10 mg/kg PQ. The lungs were removed and homogenized in lysis buffer. PKC phosphorylation was determined using antibodies against PCKpan-p, PCKα/β-p, PKCδ-p, and PKCµ-p. Immunoreactive protein bands were visualized by ECL and exposure to autoradiographic film.