Figure 4

Histologic analysis of the OA joints after treatment with GSPE in MIA-induced arthritis. The knee joints from the OA rats treated with GSPE or vehicle were stained with hematoxylin and eosin , Safranin O fast green, and toluidine blue (original magnification ×100 for hematoxylin and eosin and ×200 for Safranin O fast green and toluidine blue). (A) Two weeks after the injection of MIA and (B) 4 weeks after the injection of MIA. (C) The OA lesion was graded on a scale of 0-13 using the modified Mankin scoring system with a combined score of structure, cellular abnormalities and matrix staining. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 compared with the MIA injection group. (D) Femoral condyle 14 days after the MIA injection (original magnification ×100).