Figure 2

Effects of peptides on Ca²⁺ increase in human neutrophils. Fura-2-loaded human neutrophils were stimulated with various concentrations of GMMWAI, MMHWAM, and MMHWFM. The change in 340 nm/380 nm was monitored. The peak level of the increase in Ca²⁺ was monitored. Data are presented as means ± S.E. of four independent experiments (A-C). Fura-2-loaded human neutrophils were stimulated with 5 µM MMHWAM in the absence or presence of SK&F (10 µM), diltiazem (1 µM), nifidifin (1 µM), U-73122 (5 µM), U-73343 (5 µM), and 2A-PB (5 µM). The change in 340 nm/380 nm was monitored. The results are representative of three independent experiments (D, E). Human neutrophils were preincubated with or without 1 µg/ml of PTX for 4 h, after which fura-2 was loaded into the cells. Fura-2-loaded cells were stimulated with 5 µM MMHWAM. The peak level of the increase in Ca²⁺ was monitored. Data are presented as means ± S.E. of three independent experiments (F). ^*, P < 0.05, compared with the value obtained from the vehicle control; ^#, P < 0.05, significantly different from the -PTX control.