Figure 4
From: Identification of novel peptides that stimulate human neutrophils
Chemotactic effects of the peptides. Assays were performed using a modified Boyden chamber assay. Isolated human neutrophils (1 × 106 cells/ml of serum-free RPMI 1640) were added to the upper wells of a 96-well chemotaxis chamber, and migration across the polycarbonate membrane with 3-µm pore size was assessed after 1.5 h of incubation at 37℃ (A-C). Human neutrophils were incubated with or without 1 µg/ml of PTX for 4 h. Then, the cells were used for chemotaxis assay using 5 µM GMMWAI, MMHWAM, or MMHWFM (D-F). The numbers of migrated cells were determined by counting them in high power field (400X). The data are presented as mean ± S.E. of three independent experiments, each performed in duplicate. *P < 0.05, compared with the value obtained from the vehicle control; #P < 0.05, significantly different from the -PTX control.
