Figure 2

Syringaresinol stimulates in vivo and ex vivo NO production. (A) HUVECs were incubated with or without VEGF, syringaresinol, and L-NMA for 1 h. The intracellular levels of NO were determined by confocal laser microscopy using DAF-FM diacetate. (B) Mouse aortic rings were incubated with or without syringaresinol and L-NMA, and the NO level was determined by real-time fluorescence microscopy using DAF-FM diacetate. Data shown are the mean ± SD (n ≥ 5). *P < 0.05 and **P < 0.01 versus untreated control.