Figure 1

(A) Analysis of purified L1CAM proteins. Lane 1, purified hL1-ECD-Fc; Lane 2, hL1-ECD-S1; and Lane M, molecular weight standards. Proteins were subjected to 10% SDS-PAGE. Protein bands were visualized by Commassie blue staining. (B) Affinity determination of anti-L1CAM mAbs. Antigen-binding activities of A10-A3 (•) and cA10-A3 (▽) were determined by indirect ELISA. (C) Affinity determination of A10-A3. Antigen-binding affinities of A10-A3 (•) and chimeric A10-A3 (▽) were determined by competition ELISA. Inset represents a Klotz plot.