Figure 4
From: Differentiation of human labia minora dermis-derived fibroblasts into insulin-producing cells
Remission of diabetes in response to the transplantation of stage 3 hLMDF-derived IPCs into diabetic mice. Induced hLMDFs (stage 1-stage 3) were transplanted under the kidney capsules of streptozotocin-induced diabetic mice (blood glucose levels ≥250 mg/dl). (A) Glucose levels at the indicated time points after transplantation are given as means ± SDs. (B) Induced EGFP-hLMDF-derived IPCs (stage 3) were transplanted under the kidney capsule of streptozotocin-induced diabetic mice and blood glucose levels were monitored. (C) Intraperitoneal glucose tests were performed 28 d after transplantation. (D) 33 days after transplantation, the kidneys were removed and sectioned. EGFP-hLMDF-derived IPCs were present in the kidney capsule and were immunopositive for Pdx1, and c-peptide. However, they were immunonegative for glucagon. Tissue sections were stained with the indicated antibodies. Nuclei were stained blue with DAPI. Scale bars, 50 µm.
