Figure 1

Determination of the optimal UVB irradiation dose that would not damage HaCaT cells. HaCaT cells were starved with serum-free DMEM for 12 h and then irradiated with the indicated doses of UVB. The irradiated cells were further incubated for 12 or 24 h. The cell viability was determined by the trypan blue exclusion assay. Data indicate the means ± S.D. of three independent experiments (**P < 0.01).