Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Synergy between adiponectin and interleukin-1β on the expression of interleukin-6, interleukin-8, and cyclooxygenase-2 in fibroblast-like synoviocytes

Figure 2

The synergistic effect of adiponectin plus IL-1β on the production of IL-6, IL-8, and PGE2 in RA FLSs. Culture supernatants were evaluated for (A) IL-6, (B) IL-8, and (C) PGE2 by ELISA, as in Figure 1. The cells were used for mRNA extraction and Western blot to detect COX-2 expression. The mRNA levels were measured by real-time RT-PCR. Three independent experiments were performed in quadruplicate with FLSs from each patient. The data shown are representative of three independent experiments, and similar results were obtained from all three. Values are expressed as mean ± S.E.M. *P < 0.05 versus treatment with IL-1β (100 pg/ml) or adiponectin (10 µg/ml) alone.

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