Figure 4
The effects of the NF-κB inhibitor MG132 on the production of proinflammatory mediators such as IL-6, IL-8, and PGE2. Synovial cells (2.5 × 105 cells/60 mm dish/2 ml serum-free media) were stimulated with IL-1β (100 pg/ml) and/or adiponectin for 24 h in the presence of MG132 at concentrations of 0.25-1.0 µM. The supernatants and cells were used for ELISA (A) and real-time PCR (B), respectively. Three independent experiments were performed in quadruplicate with FLSs from each patient. The data shown are representative of three independent experiments, and similar results were obtained from all three. Values are expressed as mean ± S.E.M.
