Figure 5
Expression levels of IL-6, IL-8, PGE2, and adiponectin in synovial fluid from patients with RA or OA. Collected joint fluid from 34 patients with RA or OA was dispensed into 1-ml aliquots and treated with hyaluronidase (50 µg/ml) for 1 h at room temperature. The joint fluid was diluted with diluent buffer to achieve the proper detection range for ELISA. The levels of proteins of interest in joint fluid were measured using a commercially available ELISA kit, according to the manufacturer's instructions.
