Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro

Figure 3

Effect of the conditioned media from STO cells infected with rAd-dnIκB. Conditioned medium from STO cells infected by rAd-C was mixed with that of STO cells infected by rAd-GFP at the indicated ratio (rAd-GFP only, 9:1, 3:1, 1:1, 1:3, 1:9, and rAd-dnIκB only). ESCs were cultured in the conditioned medium for three days. (A) Morphology of representative colonies and (B) comparison of colony sizes of mESCs. The colony size (> 10 colonies/sample) was determined by measuring the colony area with the Image J program (NIH). All assays were performed in duplicate (scale bar, 25 µm).

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