Figure 4 | Experimental & Molecular Medicine

Figure 4

From: Overexpression of bone morphogenetic protein 4 in STO fibroblast feeder cells represses the proliferation of mouse embryonic stem cells in vitro

Figure 4

Overexpression of Bmp4 in STO cells infected with rAd-dnIκB inhibits mESC proliferation. (A) RT-PCR analysis of the STO feeder cells infected by rAd-GFP and rAd-dnIκB. The rAd-dnIκB infection induced overexpression of Bmp4 gene in the STO cells. GAPDH mRNA was measured as a control. (B) The relative density of genes expression was determined by dividing the density of each gene by that of GAPDH. The level of BMP4 mRNA was analyzed by using real time PCR. Results are representative of three independent experiments. (C) The number of colonies of mESCs was counted after culturing in the conditioned medium produced from STO cells plus exogenous BMP4. Addition of BMP4 resulted in a decrease in the number of colonies produced from the conditioned medium of rAd-GFP medium compared to that of rAd-dnIκB medium. (D) Morphology of mESC colonies cultured in the conditioned medium plus exogenous BMP4.

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