Figure 4

Activation of downstream signaling molecules of receptor by HRG were significantly increased in HER2-overexpressed MCF7 cells. After serum-starvation for 24 h, cells were treated with or without 50 ng/ml HRG for 30 min. The phosphorylation of HER3, HER2, ERK, JNK, Akt, c-Jun, and β-actin was determined in whole cell lysates by Western blotting. The results shown are representative of three independent experiments.