Figure 5

Differential plaque deposition patterns in the brains of Tg2576 and Tg-APP/PS1 mice were analyzed by combined application of the photographic plaque reference panels and a computer-aid image analysis method. (A-H) Photomicrographs showing +3 (A-D) or +5 (E-H) levels of plaque depositions in the prefrontal (A, C, E, G) and piriform (B, D, F, H) cortices of Tg2576 mice (A, B, E, F) and Tg-APP/PS1 mice (C, D, G, H). Tg2576 all were males aged 14.5 months (A, B) or 16 months (E, F). Tg-APP/PS1 mice all were males aged 7.5 months (C, D) or 12 months (G, H). The prefrontal cortex of the panel G is the same as the +5-prefrontal cortex in Figure 3. Scale bar; 500 µm. (I-K) The levels of plaque deposition assessed to be +3 grade (right y-axis) in the prefrontal (I) and piriform (J) cortices of Tg2576 (2576) and Tg-APP/PS1(APP/PS) mice were compared with the numbers of plaques measured by a computer-aid image analysis method (left y-axis). The total plaque areas for the prefrontal and piriform cortices of Tg2576 and Tg-APP/PS1 mice were assessed by a computer-aid method (K). Data are presented as the mean ± SEM. The numbers of animals for each group were 4-5. ^*P < 0.05; ^**P < 0.01 (Student's t-test). (L-N) The levels of plaque deposition assessed as +5 grade (right y-axis) in the prefrontal (L) and piriform (M) cortices of Tg2576 (2576) and Tg-APP/PS1 (APP/PS) mice were compared with the numbers of plaques measured by a computer-aid image analysis method (left y-axis). The total plaque areas for the prefrontal and piriform cortices of Tg2576 and Tg-APP/PS1 mice were assessed by a computer-aid method (N). Data are presented as the mean ± SEM. The numbers of animals for each group were 4-6. ^**P < 0.01 (Student's t-test).