Figure 4
From: Cyclooxygenase-2 inhibitors modulate skin aging in a catalytic activity-independent manner
NS-398 decreases but celecoxib and aspirin increase the expression of p53 and p16. NS-398 (A), celecoxib (B) and aspirin (C) were treated to the right and left side of dorsal skin of mice for 12 weeks. Skin lysates were prepared from the indicated numbered mice and western blot analysis was carried out using antibodies against p53 and p16. α-Tubulin was used as a loading control. Exposing times are variable to acquire the best quality of the data. (D) Quantitative analysis for p53 and p16 expression was done by densitometry. Data represent means ± S.E. (n = 10). **P < 0.01; compared with the corresponding vehicle-treated group.
