Figure 2

Effect of clozapine, quetiapine and ziprasidone on the expression of brown adipocyte-specific markers. Brown preadipocytes were differentiated with the induction media in the presence of (A) clozapine (■, 40 µM), (B) quetiapine (▨, 30 µM), and (C) ziprasidone (▩, 10 µM) or DMSO (□, 0.1%). At day 8 after the induction, total RNA was isolated, and the expressions of brown adipocyte markers such as PRDM16, C/EBPβ, PPARγ2, UCP-1, PGC-1α, and Cidea were determined by qRT-PCR. Relative mRNA level of each gene was normalized to the level of 36B4. The data shown are representative of three independent experiments performed in triplicate. Data are mean ± SEM. ^*P < 0.05.