Figure 3

Time-dependent effect of clozapine on the expression of brown adipocyte markers. Brown preadipocytes were differentiated for 8 days with the induction media in the presence of clozapine (■, 40 µM) or DMSO (□, 0.1%). Total RNA was isolated from the cultures at the indicated times, and the expression of brown adipocyte markers such as PRDM16, C/EBPβ, PPARγ2, UCP-1, PGC-1α, and Cidea were determined by qRT-PCR. Relative mRNA level of each gene was normalized to the level of 36B4. The data shown are representative of three independent experiments performed in triplicate. Data are mean ± SEM. ^*P < 0.05.