Figure 5
From: Inhibition of mouse brown adipocyte differentiation by second-generation antipsychotics
Dose-dependent effect of clozapine on the expression of brown adipocyte markers. Brown preadipocytes were differentiated for 8 days in induction media in the presence of indicated concentration of clozapine (■) or 0.1% DMSO (□). (A) mRNA expression. Total RNA was isolated from the cultures, and the expressions of brown adipocyte markers PRDM16, C/EBPβ, PPARγ2, UCP-1, PGC-1α, and Cidea were determined by qRT-PCR. Relative mRNA level of each gene was normalized to the levels of 36B4. Data are mean ± SEM. *P < 0.05. (B) Protein expression. Protein was prepared from the cultures at day 8, and the expression levels of UCP-1, PGC-1α and PPARγ2 were determined by immunoblot analysis. Bata-actin was used as a loading control. The data shown are representative of three independent experiments performed in triplicate.
