Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Increased arginase II activity contributes to endothelial dysfunction through endothelial nitric oxide synthase uncoupling in aged mice

Figure 1

Upregulation of ArgII activity in aged mice aortas. (A) Western blot analysis of aortas from young and aged mice. Similar levels of ArgII were found in both groups, but ArgI protein was not detectable (upper). The lower panel shows the densitometric analysis, n = 4 experiments. (B) ArgI and ArgII mRNA expression levels were analyzed by RT-PCR using GAPDH as a control. Only ArgII mRNA was detected (upper). The lower panel shows the densitometric analysis, n = 4 experiments. (C) siArgII incubation decreased arginase activity in both young and aged aortas (# vs. young, P < 0.05; * vs. young, P < 0.01; ** vs. aged, P < 0.01). (D) siArgII effectively downregulated ArgII protein expression in both young and old aortas (# vs. young, P < 0.05; * vs. aged, P < 0.01), but not with siArgI (## vs. aged, ns).

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