Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection

Figure 3

FACS analysis for binding activity of anti-EGFR human scFvs to cell surface-expressed EGFR, in which the scFvs were isolated by guided panning of mAb A13. Differential binding of scFvs to EGFR-positive A431 tumor cells and EGFR-negative HL60 cells (indicated in the panels) was analyzed. Histograms in different color codes represent the reactions of labeled secondary antibody with A431 cells only (black line), scFvs with A431 cells (red line), mAb A13 with A431 cells (red line), and scFvs and mAb A13 with HL60 cells (green lines). A, B, C, D and E denote the FACS diagram of SC414, SC 311, SC43, SC412 and mAb A13 respectively.

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