Figure 4 | Experimental & Molecular Medicine

Figure 4

From: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection

Figure 4

The arrangement of genes in expression vectors of heavy (pRC-12) and light (pKC-12) chains. PCMV denotes the cytomegalovirus promoter. Sig denotes signal peptide of human immunoglobulin. Restriction enzyme sites used for cloning are shown. The light chain expression vector contains dihydrofolate reductase (dhfr) gene (not shown in this figure) which is used for amplification of integrated genes by adjusting the cells in the increasing concentrations of methotrexate (MTX).

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