Figure 5 | Experimental & Molecular Medicine

Figure 5

From: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection

Figure 5

Competition ELISA for comparison of relative affinities of anti-EGFR IgGs. ER414 is a fully human IgG1 originated from the mAb A13 by guided panning. Binding inhibition of anti-EGFR IgGs to EGFR was analyzed on EGFR-coated plates with different concentrations of free EGFR EGFR and the curves were fitted with a 4-parameter model using SoftMaxPro software. Each symbol denotes antibodies of Cetuximab (▪), mAb A13 (□) and ER414 (•).

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