Figure 6 | Experimental & Molecular Medicine

Figure 6

From: Conversion of a murine monoclonal antibody A13 targeting epidermal growth factor receptor to a human monoclonal antibody by guided selection

Figure 6

Blocking of EGF-induced tyrosine phosphorylation of EGFR by ER414 and Cetuximab in MDA-MB-231 cells. The serum-starved cells were untreated, treated with EGF (16 nM) only, or treated with EGF (16 nM) and different concentrations of either Cetuximab or ER414 for 30 min before the Western blotting analyses. As indicated in the panel 0.7, 3.4, 33.5, 335.0 nM of Cetuximab or ER414 were included together with 335.0 nM of mAb A13. The expected size of EGFR is ~170 kDa.

Back to article page