Figure 2

Effects of enchinomycin on ET-2 mRNA and HIF-1α mRNA levels in rat ovaries. After PMSG priming for 48 h, immature rats were treated with hCG to induce ovulation and ovaries were collected from each group at 11 h after hCG. (A) The relative mRNA levels of ET-2 by real-time RT-PCR analysis. (B) The relative mRNA levels of HIF-1α by real-time RT-PCR analysis. Each value represents the mean ± SE. Student's t test was used to evaluate statistical significance of differences between two groups. The asterisk denotes significant values (P < 0.05). Ech, enchinomycin.