Figure 1

STAMP2 inhibits HBx-mediated hepatic lipid accumulation. (A) HepG2-HBx cells were transiently transfected with empty vector or mammalian STAMP2 expression vector. Oil-Red O staining was performed with spectrophotometric quantification of lipid staining at 500 nm. (B) HepG2-HBx cells were transiently transfected with empty vector or mammalian STAMP2 expression vector. Total RNA was prepared from cells, and the indicated mRNA levels were assessed by RT-PCR. (C) HepG2 cells were transiently transfected with the indicated lipogenic gene promoter constructs (ACC1-luc, FAS-luc, and SREBP1-luc) and co-transfected with empty vector or mammalian HBx and/or STAMP2 expression vectors. Cells were harvested and analyzed for luciferase activity. (D) HepG2 cells were transiently transfected with the indicated adipogenic gene promoter constructs (aP2-luc and PPARγ-luc) and co-transfected with empty vector or mammalian HBx and/or STAMP2 expression vectors. Cells were harvested and analyzed for luciferase activity. Values are expressed as the means ± SD (n = 3). ^*P < 0.05 and ^**P < 0.01 compared with mock transfectants.