Figure 2
From: Hepatic STAMP2 decreases hepatitis B virus X protein-associated metabolic deregulation
STAMP2 counteracts HBx-mediated hepatic insulin resistance. (A) HepG2-HBx cells were transiently transfected with empty vector or mammalian STAMP2 expression vector, followed by incubation with different concentrations of insulin for 1 h. Whole cell lysates were subjected to immunoblotting for PI3K-p85, phospho-PI3K-p85 (Tyr458), Akt1/2, and phospho-Akt (Ser473). (B) HepG2-HBx cells were transiently transfected with empty vector or mammalian STAMP2 expression vector, followed by incubation with different concentrations of insulin for 1 h. IRS1 protein was immunoprecipitated (IP) from total cell extracts using anti-IRS1 antibody, followed by immunoblotting with anti-phosphotyrosine antibody.
