Figure 4

STAMP2 restores insulin-mediated inhibition of gluconeogenic gene expression abrogated by HBx. (A) HepG2 cells were transfected with PEPCK (left) and G6Pase (right) promoter constructs as well as HBx and/or STAMP2 expression vectors. (B) HepG2 cells were transiently transfected with PEPCK (left) or G6Pase (right) promoter constructs as well as HBx and/or STAMP2 expression vectors in the presence or absence of insulin (100 nM). Luciferase assay was conducted to assess the promoter activities of PEPCK and G6Pase, and error bars represent the standard deviation. ^*P < 0.05 compared with the indicated control. (C) HepG2-HBx cells were transiently transfected with empty vector or GFP/STAMP2 expression plasmid in the presence or absence of insulin (100 nM). Total RNA was isolated and subjected to RT-PCR.