Figure 6

Effect of triptolide on E-cadherin expression. (A) SKOV3 and A2780 cells were incubated with triptolide at 0, 5 and 15 nM for 24 h. E-cadherin expression at protein level was evaluated by Western blotting. Tubulin was used as an internal control. (B) E-cadherin expression was suppressed (E-cadherin KD) in SKOV3 and A2780 cells by transfecting with shRNA against E-cadherin. Invasiveness of SKOV3 and A2780 cells was evaluated by counting the cells that migrated through 8 µm pore transwell membranes with matrigel. ^*P < 0.01 vs. control cells, ^#P < 0.01 vs. Triptolide-treated cells.