Figure 1 | Experimental & Molecular Medicine

Figure 1

From: The efficacy of SPA0355 in protecting β cells in isolated pancreatic islets and in a murine experimental model of type 1 diabetes

Figure 1

SPA0355 prevents cytokine-induced cell death in RINm5F cells. RINm5F cells were pre-treated with the indicated concentrations of SPA0355 for 3 h, and interleukin-1β (IL-1β) and interferon-γ (IFN-γ) were added. (a) Following a 48-h incubation, cell viability was determined using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. (b) Following a 24-h incubation, the level of nitrite production and the degree of the expression of inducible nitric oxide synthase (iNOS) mRNA and protein were determined. Each value is the mean±s.e.m. of three independent experiments (n=9). **P<0.01 vs untreated control; #P<0.05, ##P<0.01 vs cytokine treatment.

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