Figure 3 | Experimental & Molecular Medicine

Figure 3

From: The efficacy of SPA0355 in protecting β cells in isolated pancreatic islets and in a murine experimental model of type 1 diabetes

Figure 3

SPA0355 inhibits cytokine-induced Janus kinase (JAK)-signal transducer and activator of transcription (STAT) activation in RINm5F cells. RINm5F cells were pre-treated with the indicated concentrations of SPA0355 for 3 h, and interleukin-1β (IL-1β) and interferon-γ (IFN-γ) were subsequently added for 30 min. (a) Whole-cell lysates were recovered, and protein levels of suppressor of cytokine signaling (SOCSs), p-STATs and STATs were detected by western blotting. (b) Nuclear fractions were recovered, and translocation of STAT proteins to the nucleus was detected by western blotting. The data are representative of three separate experiments.

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