Figure 4 | Experimental & Molecular Medicine

Figure 4

From: The efficacy of SPA0355 in protecting β cells in isolated pancreatic islets and in a murine experimental model of type 1 diabetes

Figure 4

SPA0355 inhibits cytokine-induced activation of the nuclear factor κB (NF-κB) and Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathways in islets. Rat islets were treated with interleukin-1β (IL-1β) and interferon-γ (IFN-γ) with or without 3-h pre-treatment with 40 μM SPA0355. (a) Nitrite production and inducible nitric oxide synthase (iNOS) mRNA and protein expression levels were determined after 24 h. NF-κB-DNA binding (b) and STAT phosphorylation (c) were determined after 30 min of incubation. The results obtained with triplicate samples are expressed as the mean±s.e.m. (n=9). **P<0.01 vs untreated control; ##P<0.01 vs cytokine treatment.

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