Figure 6

SPA0355 attenuates diabetes development in non-obese diabetic (NOD) mice. Female NOD mice were injected intraperitoneally at 2-day intervals with SPA0355 (1 mg kg–1) or corn oil (n=20, each group). Diabetes development was determined by measurement of blood glucose levels (over 300 mg dl–1). (a) The percentage of diabetic mice is shown. (b) Pancreata were isolated from surviving mice at the age of 33 weeks, and pancreas sections were counterstained with hematoxylin and eosin (H&E) or labeled with insulin antibody. Insulitis was scored as described in the Materials and methods section.