Table 1 The karyotyping results of mesenchymal stromal cell lines and other adherent cell lines via classical G-banding and in situ karyotyping

From: The application of an in situ karyotyping technique for mesenchymal stromal cells: a validation and comparison study with classical G-banding

 

Classical G-banding

In situ karyotyping

Type of MSCs

Personnel 1

Personnel 2

Personnel 1

Personnel 2

Adipose-derived MSC cell line

 A-MSC-1

46,XY[20]

46,XY[20]

46,XY[20]

46,XY[20]

 A-MSC-2

46,XY[20]

46,XY[20]

46,XY[20]

46,XY[20]

 A-MSC-3

46,XY[20]

46,XY[20]

46,XY[20]

46,XY[20]

 A-MSC-4

46,XY[20]

46,XY[20]

46,XY[20]

46,XY[20]

 A-MSC-5

46,XY[11]

46,XY[14]

46,XY[20]

46,XY[20]

Cord-derived MSC cell line

 C-MSC-1

46,XY[20]

46,XY[15]

46,XY[20]

46,XY[20]

 C-MSC-2

46,XY[20]

46,XY[20]

46,XY[20]

46,XY[20]

 C-MSC-3

46,XY[20]

46,XY[20]

46,XY[20]

46,XY[20]

 C-MSC-4

46,XY[20]

46,XY[20]

46,XY[20]

46,XY[20]

 C-MSC-5

46,XY[20]

46,XY[20]

46,XY[15]

46,XY[11]

Adherent tumor cell line

 DLD-1

46,XY,dup(2)(p13p22)[20]

46,XY,dup(2)(p13p22)[20]

46,XY,dup(2)(p13p22)[20]

46,XY,dup(2)(p13p22)[20]

 HCT-116

45,X,−Y,der(10)t(10;?) (q26.1;?),der(16)t(16;?)(p13.3;?),der(18)t(18;?)(p11.32;?)[18]/46,iden,+Y[2]

45,X,−Y,der(10)t(10;?) (q26.1;?),der(16)t(16;?)(p13.3;?),der(18)t(18;?)(p11.32;?)[17]/45,idem,inv(1)(p36.1q32.1)[3]

45,X,−Y,der(10)t(10;?) (q26.1;?),der(16)t(16;?)(p13.3;?),der(18)t(18;?)(p11.32;?)[15]/45,idem,dup(5)(p13p15.1)[3]/46,idem,+Y[2]

45,X,−Y,der(10)t(10;?) (q26.1;?),der(16)t(16;?)(p13.3;?),der(18)t(18;?)(p11.32;?)[20]

  1. Abbreviation: MSC, mesenchymal stromal cell.