Figure 6

Chemokine receptor expression is regulated by rhamnogalacturonan II (RG-II) in bone marrow-derived dendritic cells (BMDCs). (a) Effect of RG-II on CCR7 (C-C motif chemokine receptor 7) expression. On day 6, BMDCs were incubated with RG-II (0.5 mg ml⁻¹) or lipopolysaccharide (LPS; 200 ng ml⁻¹) for 24 h. On day 7, phycoerythrin (PE)-conjugated CCR7 cells were analyzed by flow cytometry. Cells were gated on CD11c⁺. (b) Effect of RG-II on CCR1 and CCR7 mRNA expression. On day 6, BMDCs were incubated with RG-II (0.5 or 1 mg ml⁻¹) or were incubated with or without LPS (200 ng ml⁻¹) for 24 h. Quantitative mRNA expression was measured as described in the Materials and Methods section. * And ** indicate significant differences at P<0.05 and P<0.01, respectively, compared with unstimulated cells. The results shown are representative of three independent experiments.