Figure 5
Daily and circadian patterns of water intake and urine excretion in WT and PDK mice. (a) Daily patterns of water intake and urine excretion. WT and PDK mice were housed individually in metabolic cages. These animals were further entrained to a 12-h light/12-h dark (LD 12:12) photoperiodic cycle with lights on at 0800 hours for a week. On the 8th day, the water intake and urine excretion were determined at 2-h intervals throughout a day and are shown as line graphs. Then, the 12-h day, 12-h night and 24-h all-day amounts were pooled and are shown as bar graphs on the right side. (b) Circadian patterns of water intake and urine excretion. To determine the circadian patterns, the lights were turned off on the 9th day. Two days after the lights were turned off, the water intake and urine excretion under the DD condition were determined at 2-h intervals throughout a circadian cycle and are shown as line graphs. Then, the 12-h subjective day, 12-h subjective night and 24-h all-day amounts were pooled and are shown as bar graphs on the right side (b). *P<0.05.LD, light/dark; DD, constant darkness; PDK, double knockout of Per1 and Per2 genes, WT, wild type.
