Figure 2

LR11 and CD9 associate on the cell surface of PMA/THP-1. (a) The levels of sLR11 and the cellular expression of LR11 or CD9 in undifferentiated THP-1, differentiated PMA/THP-1 and CCRF-SB cells, as analyzed by western blot analysis. For analysis of the levels of cellular protein, the cells were cultured with serum-containing media. For analysis of sLR11 in the culture supernatant, the cells were cultured with fresh serum-free media for 24 h. The collected media were concentrated before analysis. (b) Confocal microscopy displaying the colocalization and interaction of LR11 and CD9 in PMA/THP-1. After THP-1 cells were incubated with 20 ng ml⁻¹ PMA for 24 h, the attached cells were stained with anti-LR11 and anti-CD9 antibodies. Samples were analyzed by confocal microscopy.