Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Different uptake of gentamicin through TRPV1 and TRPV4 channels determines cochlear hair cell vulnerability

Figure 1

Hair cell death caused by gentamicin in a time- and dose-dependent manner. (A) Cochlear explant cultures from postnatal day 3 rats were maintained in the absence (a, c) or presence (b, d) of 300 μM gentamicin for 24 h. Cultures were stained with phalloidin– tetramethylrhodamine isothiocyanate (TRITC; a, b) and 4′,6-diamidino-2-phenylindole (DAPI; c, d) and observed under a fluorescent microscope. Outer hair cells (OHCs): arrow, inner hair cells (IHCs): arrowhead, and Hensen’s cells: star. (B) Quantitative analysis of OHC loss in explants treated for 24, 36 and 48 h with various doses (50, 100, 200, 300, 400, 500, 600 and 700 μM) of gentamicin. The percentage of hair cells missing at various gentamicin doses was significantly different from that of the control. Data are mean±s.d. of three samples. *P<0.05 and **P<0.01 by one-way analysis of variance (ANOVA), compared with each turn of control group not treated with gentamicin.

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