Figure 8
Effect of transient receptor potential vanilloid (TRPV) channel inhibitors on neuromast hair cell damage in gentamicin-treated zebrafish. At 5 day post fertilization (dpf), zebrafish larvae were treated with 300 μM for 1 h and allowed to recover for 1 h. (a) Hair cells labeled with YO-PRO-1. The scale bar in (a) is 5 μm and applies to other panels also. (b) Hair cells are labeled with 2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide (DASPEI). Mean hair cell survival was estimated using DASPEI scoring from 10 neuromasts per larvae (**P<0.01, one-way analysis of variance (ANOVA)). (c) The 5 dpf, larvae were treated with 300 μM gentamicin-conjugated Texas Red (GTTR) for 15 min and allowed to recover for 30 min. Then, larvae were further stained with YO-PRO-1 at 1 μM for 30 min. Arrow in (c) indicates GTTR uptake in hair cells.
