Table 1 Sequences and accession numbers for primers used in real-time RT-PCR

From: Inhibition of Janus activated kinase-3 protects against myocardial ischemia and reperfusion injury in mice

Gene

Sequences for primers

Accession no.

ICAM-1

FOR: 5′-AACAGTTCACCTGCACGGAC-3′

NM_010493

 

REV: 5′-GTCACCGTTGTGATCCCTG-3′

 

MCP-1

FOR: 5′-ATTGGGATCATCTTGCTGGT-3′

NM_011333

 

REV: 5′-CCTGCTGTTCACAGTTGCC-3′

 

IL-8

FOR: 5′-CAGCTGCCTTAACCCCATCA-3′

NM_009909

 

REV: 5′-TGAGAAGTCCATGGCGAAATT-3′

 

TNF-α

FOR: 5′-AGGGTCTGGGCCATAGAACT-3′

NM_013693

 

REV: 5′-CCACCACGCTCTTCTGTCTAC-3′

 

GAPDH

FOR: 5′-CGTCCCGTAGACAAAATGGT-3′

NM_008084

 

REV: 5′-TTGATGGCAACAATCTCCAC-3′

 
  1. Abbreviations: FOR, forward; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ICAM-1, intercellular adhesion molecule-1; IL-8, interleukin-8; MCP-1, monocyte chemoattractant protein-1; REV, reverse; RT-PCR, reverse transcriptase-PCR; TNF-α, tumor necrosis factor-α.
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