Figure 3
From: Reactive oxygen species regulate context-dependent inhibition of NFAT5 target genes
Mannitol induces NFAT5 activity but reduces LPS-induced IL-6 production. (a and b) Dose-dependent increases in NFAT5 reporter activity after treatment with mannitol. Mannitol (100, 200 and 400 mM) was added to RAW 264.7 macrophages harboring the NFAT5 reporter gene for 24 h. The bar graph in b shows the mean±s.d. of three independent experiments. (c) Suppression of LPS-triggered IL-6 production by cotreatment with mannitol. RAW 264.7 macrophages were stimulated with LPS (5 μg ml−1) for 24 h in the presence of mannitol. IL-6 levels in the supernatant were determined by enzyme-linked immunosorbent assay. *P<0.01. The degree of cell viability was assessed by an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and presented as the percentage of the cell viability of treated samples to the viability of control untreated cells.
