Figure 2
From: Signaling mechanisms of glucose-induced F-actin remodeling in pancreatic islet β cells
Cortical filamentous actin (F-actin) remodeling in β cells regulates insulin granule exocytosis. (a) Schematic of a pancreatic β cell depicts how readily releasable pool (RRP) granules are docked at the membrane and reserve pool granules are in a more intracellular storage pool. Cortical F-actin can regulate access of reserve pool granules to the readily releasable pool. (b) Microscopic analysis of β cell-actin remodeling is often performed on cells attached to coverslips (a very different environment from the three-dimensional islet architecture) where it is important to note that focal adhesions/stress fibers form at the cell attachment interface. Conversely, cortical F-actin is visualized at the cell perimeter when focused at the mid plane of the cell. This distinction is particularly important in differentiating cortical F-actin remodeling from other types of actin remodeling.
