Figure 2
From: Phospholipase D inhibitor enhances radiosensitivity of breast cancer cells
Phospholipase D (PLD) inhibition promotes ionizing radiation (IR)-induced cell death. (a) MDA-MB-231 cells were treated with 10 μM of PLD inhibitor for 4 h and/or exposed to 5 Gy radiation. After 48 h, the cells were stained with 4',6-diamidino-2-phenylindole (DAPI) and observed by fluorescence microscopy. The live cells were quantitated. (b) The cells were treated with 10 μM PLD inhibitor for 4 h and/or exposed to 5 Gy radiation. After 48 h, the cells were stained with Annexin V and 7-amino-actinomycin (7-AAD) and analyzed by flow cytometry. Data are expressed as the mean±s.d. for three experiments. (c) The cells were treated with PLD inhibitor and/or 5 Gy radiation. The cell lysates were analyzed by western blot using the indicated antibodies. The band intensity was quantified. These blots are representative of the results obtained from three experiments.
