Figure 1
From: U6 is unsuitable for normalization of serum miRNA levels in patients with sepsis or liver fibrosis
RNU6B (U6) levels display high variability in murine serum. (a, c) Serum U6 levels were analyzed by quantitative real-time PCR (qPCR) in C57Bl/6 mice before and 24 h after the cecal ligation and puncture (CLP) surgery and revealed a very high variability of U6 levels in the different mice (n=14 per group). Levels of spiked-in SV40-RNA was determined by qPCR in samples from C57Bl/6 mice before and 24 h after CLP surgery, revealing a very low variability between the different samples. (b, d) The variability of U6 values and SV40 values was statistically compared, revealing a significantly higher variability in U6 values compared with that of SV40. (e) Raw U6 Ct values were compared between C57Bl/6 mice before and 24 h after CLP surgery, revealing no significant differences between both groups. (f) U6 serum levels were compared between C57Bl/6 mice before and 24 h after CLP surgery, revealing no significant differences between both groups also when data were normalized using SV40. **P<0.01, ***P<0.001.
