Figure 2
From: U6 is unsuitable for normalization of serum miRNA levels in patients with sepsis or liver fibrosis
RNU6B (U6) levels display high variability in human serum. (a) Serum U6 levels were analyzed by quantitative real-time PCR (qPCR) in healthy donors and revealed a high interindividual variability (n=44). (b) Levels of spiked-in SV40-RNA were determined by qPCR in samples from healthy donors, revealing a very low variability between the different samples. (c) The variability of U6 values and SV40 values was statistically compared, revealing a significantly higher variability in U6 values compared with that of SV40. ***P<0.001.
