Figure 3
From: U6 is unsuitable for normalization of serum miRNA levels in patients with sepsis or liver fibrosis
Levels of circulating RNU6B (U6) are elevated in critical illness. (a) Serum U6 levels at admission to the intensive care unit (ICU) were analyzed by quantitative real-time PCR (qPCR) and revealed a very high variability between different individuals (n=203). (b) Levels of spiked-in SV40-RNA were determined by qPCR in samples from patients with critical illness, revealing a very low variability between the different samples. (c) The variability of U6 values and SV40 values was statistically compared, revealing a significantly higher variability in U6 values compared with that of SV40. (d) U6 serum levels were compared between critically ill patients and healthy donors, revealing significantly higher levels of U6 in the patients’ group (corresponding to lower Ct values). (e) U6 serum levels were compared between critically ill patients and healthy donors, revealing significantly higher levels of U6 in the patients’ group also when data were normalized using SV40. (f) U6 serum levels were analyzed with respect to the different etiologies of critical illness. Abd, abdominal; Cirrh, cirrhosis; C.p., cardiopulmonary; Uro, urological. ***P<0.001.
