Figure 5
Decreased background staining with new immunoblot (IB) method coupled with application of magnetic beads in immunoprecipitation (IP). Immune complexes were precipitated either with protein A agarose beads (left panels) or cotinine-crosslinked magnetic beads (middle and right panels). (a) Blots were incubated with anti-C5 × anti-cotinine bispecific tandem scFv-Fc fusion protein and then probed with either horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Fc-specific) antibody (left and middle panels) or cotinine–HRP (right panel). (b) The control blots were incubated with secondary probes only, HRP-conjugated goat anti-rabbit IgG (Fc-specific) antibody (left and middle panels) or cotinine–HRP (right panel).
