Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Aspirin-induced Bcl-2 translocation and its phosphorylation in the nucleus trigger apoptosis in breast cancer cells

Figure 2

Aspirin-induced translocation of B-cell lymphoma 2 (Bcl-2) in MCF-7 cells. (a) The effect of aspirin on endogenous Bcl-2. MCF-7 cells were exposed to 5 mM aspirin for several time intervals as indicated. The distribution of Bcl-2 was visualized after immunostaining with anti-Bcl-2 and anti-FKBP38 antibodies. (b) MCF-7 cells were exposed to 5 mM aspirin for 24 h. Nuclear and cytoplasmic fractions were prepared as described in the MATERIALS AND METHODS and subjected to immunoblotting with the indicated antibodies. Lamin A, α-tubulin and FKBP12 were used as nucleus- and cytoplasm-specific controls. (c) FKBP38 and Bcl-2 are downregulated in mitochondrial fractions obtained from cells exposed to 5 mM aspirin for 24 h. C, cytosolic fraction, N, nuclear fraction, M, mitochondrial fraction.

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