Figure 3
Histological images of livers from an oleuropein-supplemented HFD (OSD) and high-fat diet (HFD) groups after hematoxylin and eosin (H&E) and Masson’s trichrome (MT) staining. (A) H&E staining (panels A–D; × 150 object, bar=100 μm, black box; × 400 object, scale bar=25 μm); (B) MT staining (panels A–D; × 200 object, scale bar=50 μm). H&E and MT stains were applied to formalin-fixed paraffin sections. HFD, 60% HFD fed mice. OSD, 0.05% oleuropein mixed with 60% HFD mice. HFD mice were fed the HFD for 9 or 12 months (panels a, b). The OSD mice were fed oleuropein in conjunction with a HFD after 6 months of the HFD alone (panels c, d). Inflammatory cells are indicated by yellow arrows. (C) The percentage of fibrosis in the OSD group after 3 months of oleuropein treatment (*P<0.05). (D) The percentage of fibrosis in the OSD group after 6 months of treatment (*P<0.05).
