Figure 1
Pretreatment with bucillamine protects HEI-OC1 cells from cisplatin-induced apoptosis. (a) Cells were pretreated with various concentrations of bucillamine for 1 h followed by the addition of 20 μM cisplatin for 30 h, and cell viability was measured by MTT assays. (b) Genomic DNA isolated from cells, treated as indicated, was subject to 1.5% agarose gel electrophoresis. (c) Cells were stained with DAPI and observed under a fluorescence microscope to detect morphological changes in the nuclei. (d) Cells were treated with 2 mM bucillamine and the cell cycle was analyzed, according to sub-G0/G1 fraction ratio by flow cytometry. The results are shown as the mean % (s.d.) of triplicate experiments. * Indicates a significant difference between cells treated with medium alone, cisplatin or cisplatin plus bucillamine (P<0.01 by one-way analysis of variance test).
